What Is the Double Marker Test?

The Double Marker Test is a first-trimester blood test done between 9–13 weeks of pregnancy.
It measures two substances in the mother’s blood:

  • Free β-hCG (Human Chorionic Gonadotropin)
  • PAPP-A (Pregnancy-Associated Plasma Protein-A)

It is usually combined with the NT scan (Nuchal Translucency scan) to assess the baby’s risk for certain chromosomal conditions.

Benefits of the Double Marker Test

Early Screening for Chromosomal Conditions


It helps estimate the risk of:

  • Down syndrome
  • Edwards syndrome
  • Patau syndrome

This is a screening test, not a diagnostic test. It tells you the probability (risk), not a confirmed diagnosis.


Early Detection = More Options

Because it is done in the first trimester, it gives:

  • More time for additional testing (if needed)
  • Time for informed decision-making
  • Early specialist consultation

Non-Invasive and Safe

  • Requires only a simple blood sample
  • No risk to mother or baby
  • No radiation

More Accurate When Combined With NT Scan


When combined with:

  • Maternal age
  • NT scan measurement
  • Blood test values

It significantly improves screening accuracy compared to age alone.

How Results Are Reported


Results are usually given as a risk ratio, for example:

  • 1 in 1,000 → Low risk
  • 1 in 100 → Higher risk

Your doctor will interpret it based on your age and scan findings


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